Nanoliter Multiplex PCR Arrays on a SlipChip
نویسندگان
چکیده
منابع مشابه
Nanoliter multiplex PCR arrays on a SlipChip.
The SlipChip platform was tested to perform high-throughput nanoliter multiplex PCR. The advantages of using the SlipChip platform for multiplex PCR include the ability to preload arrays of dry primers, instrument-free sample manipulation, small sample volume, and high-throughput capacity. The SlipChip was designed to preload one primer pair per reaction compartment and to screen up to 384 diff...
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We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an array format served as individual PCR microreactors. A simple contact printing technique was developed to generate a large ...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2010
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac1007249